Journal article
The use of miRNA microarrays for the analysis of cancer samples with global miRNA decrease
D Wu, Y Hu, S Tong, BRG Williams, GK Smyth, MP Gantier
RNA | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2013
Abstract
Recent studies have established that mutations or deletions in microRNA (miRNA) processing enzymes resulting in a global decrease of miRNA expression are frequent across cancers and can be associated with a poorer prognosis. While very popular in miRNA profiling studies, it remains unclear whether miRNA microarrays are suited or not to accurately detecting global miRNA decreases seen in cancers. In this work, we analyzed the miRNA profiles of samples with global miRNA decreases using Affymetrix miRNA microarrays following the inducible genetic deletion of Dicer1. Surprisingly, up to a third of deregulated miRNAs identified upon Dicer1 depletion were found to be up-regulated following standar..
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Grants
Awarded by Australian National Health and Medical Research Council
Funding Acknowledgements
We thank Mark Van der Hoek (Adelaide Microarray Centre, Adelaide, Australia) for his help with RNA labeling and Affymetrix microarray processing, and Frances Cribbin for editorial assistance. This work was supported by grants from the Australian National Health and Medical Research Council (1022144 to M. P. G., 1036541 to D. W., 1006590 to B. R. G. W., 490970 to S. T., and 490036 to G. K. S.) and the Victorian Government's Operational Infrastructure Support Program.